Generating Antibodies to Contrast Aβ Deposition
Research area: Protein Folding and Quality Control
Group leaders: Maurizio Molinari
Status: In progress
We engineered antibodies to be expressed in a transgenic mice model for Alzheimer’s disease from a single open reading frame encoding the heavy and light chain of a specific antibody to the N-terminus of the Aβ peptide linked by the mouth and foot virus peptide 2A (to promote equimolar expression of light and heavy chains that are efficiently processed and assembled in the ER of transgenic cells). The in situ production of the antibody substantially reduced Aβ formation and aggregation compared to age-matched, mock-treated APP23 mice.
Paganetti et al., Neurobiol of Aging. 2013; 34:2866-2878.