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Institute for Research in Biomedicine
Istituto di Ricerca in Biomedicina

Via Vincenzo Vela 6 - CH-6500 Bellinzona
Tel. +41 91 820 0300 - Fax +41 91 820 0302 - info [at] irb [dot] usi [dot] ch

Gene Expression and Protein Production Facility (GEPP)

Manager: Laurent Perez

Researchers:

The IRB has developed relevant competence in the field of protein-protein and antigen-antibody interaction applied to the study of inflammatory and infectious diseases.

The Gene Expression and Protein Purification facility (GEPP) helps IRB researchers with cellular biochemistry, protein expression and production but also develops and evaluates new techniques for protein production and purification. Assistance and information are offered to all IRB research laboratories. The facility is equipped for small to medium scale purification from cell free system, bacteria, insect and mammalian cell lines. It is currently specializing in the use of the baculovirus expression system and mammalian expression, especially for production of multimeric protein complexes.  Depending upon the level of expression of a given protein, quantity ranging from milligrams to grams can be produced using resources at the facility. Finally, the facility is centralizing stocks of a large number of expression vectors for insect and mammalian cells.

Equipment available in the GEPP facility : ÄKTA™purifier, tangential flow filtration system from Millipore.

This facility is enhanced by collaboration with the Swiss Supercomputer Center located in Ticino as well as with numerous international institutions.