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Institute for Research in Biomedicine
Istituto di Ricerca in Biomedicina

Via Vincenzo Vela 6 - CH-6500 Bellinzona
Tel. +41 91 820 0300 - Fax +41 91 820 0302 - info [at] irb [dot] usi [dot] ch

Fabio Grassi, MD, PhD

T Cell Development

Group Leader

Via Vela, 6
6500 Bellinzona, Switzerland

Fabio Grassi
Bio

Fabio Grassi earned his degree in Medicine at the University of Pavia in 1985 and a Ph.D. in Microbiology at the University of Milan in 1993. He was a Anna Villa Rusconi fellow at the University of Umeä in Sweden (1988), post-doctoral fellow at the Institut Pasteur in Paris (1989-1993), assistant professor at San Raffaele Scientific Institute in Milan (1994-1998), Marie Curie fellow at Hôpital Necker in Paris (1998-2000) and Special Fellow of the Leukemia & Lymphoma Society at Dana Farber Cancer Institute, Harvard Medical School in Boston (2000-2002). He is full professor of Biology at the Medical School of the University of Milan. The research in the lab is focused on the purinergic control of T cell response. At the moment, particular efforts are dedicated to defining the role of extracellular ATP and P2X7 receptor in regulating mucosal adaptive response as well as mutualism with intestinal commensals.

Projects
Regulation of mucosal immunity in the gut by purinergic P2X7 receptor
Development of an implantable system to monitor inflammation or metabolism
Ectonucleotidase activity in class switch recombination of murine B cells
Purinergic antagonism in muscular dystrophy
Purinergic P2X7 receptor in type 1 diabetes
P2X7 receptor in T cell lineage choice and shaping of γδ cells
Induced thymus development as a therapeutic approach in Omenn Syndrome
Publications
Modulation of innate and adaptive immunity by P2X ion channels
F. Di Virgilio, A. C. Sarti, F. Grassi
in Curr Opin Immunol (2018) vol. 52 pp51-59
TRPM7 kinase activity is essential for T cell colonization and alloreactivity in the gut
A. Romagnani, V. Vettore, T. Rezzonico-Jost, S. Hampe, E. Rottoli, W. Nadolni, M. Perotti, M. A. Meier, C. Hermanns, S. Geiger, G. Wennemuth, C. Recordati, M. Matsushita, S. Muehlich, M. Proietti, V. Chubanov, T. Gudermann, F. Grassi, S. Zierler
in Nat Commun (2017) vol. 8 pp1917
Immunological phenotype of the murine Lrba knockout
L. Gamez-Diaz, J. Neumann, F. Jager, M. Proietti, F. Felber, P. Soulas-Sprauel, L. Perruzza, F. Grassi, T. Kogl, P. Aichele, M. Kilimann, B. Grimbacher, S. Jung
in Immunol Cell Biol (2017) vol. 95 pp789-802
T Follicular Helper Cells Promote a Beneficial Gut Ecosystem for Host Metabolic Homeostasis by Sensing Microbiota-Derived Extracellular ATP
L. Perruzza, G. Gargari, M. Proietti, B. Fosso, A. M. D'Erchia, C. E. Faliti, T. Rezzonico-Jost, D. Scribano, L. Mauri, D. Colombo, G. Pellegrini, A. Moregola, C. Mooser, G. Pesole, M. Nicoletti, G. D. Norata, M. B. Geuking, K. D. McCoy, S. Guglielmetti, F. Grassi
in Cell Rep (2017) vol. 18 pp2566-2575
Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
V. Cecchinato, E. Bernasconi, R. F. Speck, M. Proietti, U. Sauermann, G. D'Agostino, G. Danelon, T. Rezzonico Jost, F. Grassi, L. Raeli, F. Schoni-Affolter, C. Stahl-Hennig, M. Uguccioni, H. I. V. Cohort Study Swiss
in J Immunol (2017) vol. 198 pp184-195
In-Vivo Validation of Fully Implantable Multi-Panel Devices for Remote Monitoring of Metabolism
C. Baj-Rossi, A. Cavallini, E. G. Kilinc, F. Stradolini, T. Rezzonico Jost, M. Proietti, G. De Micheli, F. Grassi, C. Dehollain, S. Carrara
in IEEE Trans Biomed Circuits Syst (2016) vol. 10 pp955-962
RAGs and BUGS: an alliance for autoimmunity
R. Rigoni, F. Grassi, A. Villa, B. Cassani
in Gut Microbes (2016) vol. 7 pp503-511
Role of CXCR4-mediated bone marrow colonization in CNS infiltration by T cell acute lymphoblastic leukemia
T. R. Jost, C. Borga, E. Radaelli, A. Romagnani, L. Perruzza, L. Omodho, G. Cazzaniga, A. Biondi, S. Indraccolo, M. Thelen, G. Te Kronnie, F. Grassi
in J Leukoc Biol (2016) vol. 99 pp1077-87
Intestinal microbiota sustains inflammation and autoimmunity induced by hypomorphic RAG defects
R. Rigoni, E. Fontana, S. Guglielmetti, B. Fosso, A. M. D'Erchia, V. Maina, V. Taverniti, M. C. Castiello, S. Mantero, G. Pacchiana, S. Musio, R. Pedotti, C. Selmi, J. R. Mora, G. Pesole, P. Vezzoni, P. L. Poliani, F. Grassi, A. Villa, B. Cassani
in J Exp Med (2016) vol. 213 pp355-75
Ectonucleotidase activity and immunosuppression in astrocyte-CD4 T cell bidirectional signaling
F. Filipello, D. Pozzi, M. Proietti, A. Romagnani, S. Mazzitelli, M. Matteoli, C. Verderio, F. Grassi
in Oncotarget (2016) vol. 7 pp5143-56
Enhancement of Muscle T Regulatory Cells and Improvement of Muscular Dystrophic Process in mdx Mice by Blockade of Extracellular ATP/P2X Axis
E. Gazzerro, S. Baldassari, S. Assereto, F. Fruscione, A. Pistorio, C. Panicucci, S. Volpi, L. Perruzza, C. Fiorillo, C. Minetti, E. Traggiai, F. Grassi, C. Bruno
in Am J Pathol (2015) vol. 185 pp3349-60
ATP-Gated Ionotropic P2X7 Receptor Controls Follicular T Helper Cell Numbers in Peyer's Patches to Promote Host-Microbiota Mutualism
M. Proietti, V. Cornacchione, T. Rezzonico Jost, A. Romagnani, C. E. Faliti, L. Perruzza, R. Rigoni, E. Radaelli, F. Caprioli, S. Preziuso, B. Brannetti, M. Thelen, K. D. McCoy, E. Slack, E. Traggiai, F. Grassi
in Immunity (2014) vol. 41 pp789-801
LPS Nephropathy in Mice Is Ameliorated by IL-2 Independently of Regulatory T Cells Activity
R. Bertelli, A. Di Donato, M. Cioni, F. Grassi, M. Ikehata, A. Bonanni, M. P. Rastaldi, G. M. Ghiggeri
in PLoS One (2014) vol. 9 ppe111285
Full fabrication and packaging of an implantable multi-panel device for monitoring of metabolites in small animals
C. Baj-Rossi, E. G. Kilinc, S. S. Ghoreishizadeh, D. Casarino, T. R. Jost, C. Dehollain, F. Grassi, L. Pastorino, G. De Micheli, S. Carrara
in IEEE Trans Biomed Circuits Syst (2014) vol. 8 pp636-47
Enhancing chemotherapy efficacy in Pten-deficient prostate tumors by activating the senescence-associated antitumor immunity
A. Toso, A. Revandkar, D. Di Mitri, I. Guccini, M. Proietti, M. Sarti, S. Pinton, J. Zhang, M. Kalathur, G. Civenni, D. Jarrossay, E. Montani, C. Marini, R. Garcia-Escudero, E. Scanziani, F. Grassi, P. P. Pandolfi, C. V. Catapano, A. Alimonti
in Cell Rep (2014) vol. 9 pp75-89
Rag Defects and Thymic Stroma: Lessons from Animal Models
V. Marrella, P. L. Poliani, L. D. Notarangelo, F. Grassi, A. Villa
in Front Immunol (2014) vol. 5 pp407
Tumour-infiltrating Gr-1+ myeloid cells antagonize senescence in cancer
D. Di Mitri, A. Toso, J. J. Chen, M. Sarti, S. Pinton, T. R. Jost, R. D'Antuono, E. Montani, R. Garcia-Escudero, I. Guccini, S. Da Silva-Alvarez, M. Collado, M. Eisenberger, Z. Zhang, C. Catapano, F. Grassi, A. Alimonti
in Nature (2014) vol. 515 pp134-7
Continuous monitoring of Naproxen by a cytochrome P450-based electrochemical sensor
C. Baj-Rossi, T. Rezzonico Jost, A. Cavallini, F. Grassi, G. De Micheli, S. Carrara
in Biosens Bioelectron (2014) vol. 53 pp283-7
Dependence of immunoglobulin class switch recombination in B cells on vesicular release of ATP and CD73 ectonucleotidase activity
F. Schena, S. Volpi, C. E. Faliti, F. Penco, S. Santi, M. Proietti, U. Schenk, G. Damonte, A. Salis, M. Bellotti, F. Fais, C. Tenca, M. Gattorno, H. Eibel, M. Rizzi, K. Warnatz, M. Idzko, C. K. Ayata, M. Rakhmanov, T. Galli, A. Martini, M. Canossa, F. Grassi, E. Traggiai
in Cell Rep (2013) vol. 3 pp1824-31
Remote system for monitoring animal models with single-metabolite bio-nano-sensors
S. Carrara, L. Bolomey, C. Boero, A. Cavallini, E. Meurville, G. De Micheli, T. Rezzonico Jost, M. Proietti, F. Grassi
in IEEE Sensors Journal (2013) vol. 13 pp1018-1024
Effect of the purinergic inhibitor oxidized ATP in a model of islet allograft rejection
A. Vergani, C. Fotino, F. D'Addio, S. Tezza, M. Podetta, F. Gatti, M. Chin, R. Bassi, R. D. Molano, D. Corradi, R. Gatti, M. E. Ferrero, A. Secchi, F. Grassi, C. Ricordi, M. H. Sayegh, P. Maffi, A. Pileggi, P. Fiorina
in Diabetes (2013) vol. 62 pp1665-75
Long-Term Heart Transplant Survival by Targeting the Ionotropic Purinergic Receptor P2X7
A. Vergani, S. Tezza, F. D'Addio, C. Fotino, K. Liu, M. Niewczas, R. Bassi, R. D. Molano, S. Kleffel, A. Petrelli, A. Soleti, E. Ammirati, M. Frigerio, G. Visner, F. Grassi, M. E. Ferrero, D. Corradi, R. Abdi, C. Ricordi, M. H. Sayegh, A. Pileggi, P. Fiorina
in Circulation (2013) vol. 127 pp463-75
Phenotypic analysis and isolation of murine hematopoietic stem cells and lineage-committed progenitors
M. Frascoli, M. Proietti, F. Grassi
in J Vis Exp (2012)
Anti-CD3{varepsilon} mAb improves thymic architecture and prevents autoimmune manifestations in a mouse model of Omenn syndrome: therapeutic implications
V. Marrella, P. L. Poliani, E. Fontana, A. Casati, V. Maina, B. Cassani, F. Ficara, M. Cominelli, F. Schena, M. Paulis, E. Traggiai, P. Vezzoni, F. Grassi, A. Villa
in Blood (2012) vol. 120 pp1005-14
Purinergic P2X7 Receptor Drives T Cell Lineage Choice and Shapes Peripheral gammadelta Cells
M. Frascoli, J. Marcandalli, U. Schenk, F. Grassi
in J Immunol (2012) vol. 189 pp174-80
Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells
M. T. Pallotta, C. Orabona, C. Volpi, C. Vacca, M. L. Belladonna, R. Bianchi, G. Servillo, C. Brunacci, M. Calvitti, S. Bicciato, E. M. Mazza, L. Boon, F. Grassi, M. C. Fioretti, F. Fallarino, P. Puccetti, U. Grohmann
in Nat Immunol (2011) vol. 12 pp870-8
Pharmacologic P2X purinergic receptor antagonism in the treatment of collagen-induced arthritis
V. Ardissone, E. Radaelli, P. Zaratin, M. Ardizzone, C. Ladel, M. Gattorno, A. Martini, F. Grassi, E. Traggiai
in Arthritis Rheum (2011) vol. 63 pp3323-32
ATP Inhibits the Generation and Function of Regulatory T Cells Through the Activation of Purinergic P2X Receptors
U. Schenk, M. Frascoli, M. Proietti, R. Geffers, E. Traggiai, J. Buer, C. Ricordi, A. M. Westendorf, F. Grassi
in Sci Signal (2011) vol. 4 ppra12
Cell-autonomous regulation of hematopoietic stem cell cycling activity by ATP
A. Casati, M. Frascoli, E. Traggiai, M. Proietti, U. Schenk, F. Grassi
in Cell Death Differ (2011) vol. 18 pp396-404
Purinergic control of neutrophil activation
F. Grassi
in J Mol Cell Biol (2010) vol. 2 pp176-7
Selective preservation of bone marrow mature recirculating but not marginal zone B cells in murine models of chronic inflammation
E. Traggiai, A. Casati, M. Frascoli, S. Porcellini, M. Ponzoni, F. Sanvito, L. Leng, R. Bucala, L. Moretta, F. Grassi
in PLoS One (2010) vol. 5 ppe11262
Homeostatic expansion of autoreactive immunoglobulin-secreting cells in the Rag2 mouse model of Omenn syndrome
B. Cassani, P. L. Poliani, V. Marrella, F. Schena, A. V. Sauer, M. Ravanini, D. Strina, C. E. Busse, S. Regenass, H. Wardemann, A. Martini, F. Facchetti, M. van der Burg, A. G. Rolink, P. Vezzoni, F. Grassi, E. Traggiai, A. Villa
in J Exp Med (2010) vol. 207 pp1525-40
Hierarchy of immunosuppressive strength among myeloid-derived suppressor cell subsets is determined by GM-CSF
L. Dolcetti, E. Peranzoni, S. Ugel, I. Marigo, A. Fernandez Gomez, C. Mesa, M. Geilich, G. Winkels, E. Traggiai, A. Casati, F. Grassi, V. Bronte
in Eur J Immunol (2010) vol. 40 pp22-35
Purinergic control of T cell activation by ATP released through pannexin-1 hemichannels
U. Schenk, A. M. Westendorf, E. Radaelli, A. Casati, M. Ferro, M. Fumagalli, C. Verderio, J. Buer, E. Scanziani, F. Grassi
in Sci Signal (2008) vol. 1 ppra6
Recombinase-deficient T cell development by selective accumulation of CD3 into lipid rafts
D. Ferrera, M. Panigada, S. Porcellini, F. Grassi
in Eur J Immunol (2008) vol. 38 pp1148-56
Of Omenn and mice
V. Marrella, P. L. Poliani, C. Sobacchi, F. Grassi, A. Villa
in Trends Immunol (2008) vol. 29 pp133-40
A hypomorphic R229Q Rag2 mouse mutant recapitulates human Omenn syndrome
V. Marrella, P. L. Poliani, A. Casati, F. Rucci, L. Frascoli, M. L. Gougeon, B. Lemercier, M. Bosticardo, M. Ravanini, M. Battaglia, M. G. Roncarolo, M. Cavazzana-Calvo, F. Facchetti, L. D. Notarangelo, P. Vezzoni, F. Grassi, A. Villa
in J Clin Invest (2007) vol. 117 pp1260-9
Notch1-dependent lymphomagenesis is assisted by but does not essentially require pre-TCR signaling
A. F. Campese, A. I. Garbe, F. Zhang, F. Grassi, I. Screpanti, H. von Boehmer
in Blood (2006) vol. 108 pp305-10
Regulation of peripheral T cell activation by calreticulin
S. Porcellini, E. Traggiai, U. Schenk, D. Ferrera, M. Matteoli, A. Lanzavecchia, M. Michalak, F. Grassi
in J Exp Med (2006) vol. 203 pp461-71
Involvement of Prep1 in the alphabeta T-cell receptor T-lymphocytic potential of hematopoietic precursors
D. Penkov, P. Di Rosa, L. Fernandez Diaz, V. Basso, E. Ferretti, F. Grassi, A. Mondino, F. Blasi
in Mol Cell Biol (2005) vol. 25 pp10768-81
Improved thymopoietic potential in aviremic HIV infected individuals treated with HAART by intermittent IL-2 administration
S. Porcellini, G. Vallanti, S. Nozza, G. Poli, A. Lazzarin, G. Tambussi, A. G. Siccardi, F. Grassi
in AIDS (2003) vol. 17 pp1621-30
Thymic selection revisited: how essential is it?
H. von Boehmer, I. Aifantis, F. Gounari, O. Azogui, L. Haughn, I. Apostolou, E. Jaeckel, F. Grassi, L. Klein
in Immunol Rev (2003) vol. 191 pp62-78
Constitutive endocytosis and degradation of the pre-T cell receptor
M. Panigada, S. Porcellini, E. Barbier, S. Hoeflinger, P. A. Cazenave, H. Gu, H. Band, H. von Boehmer, F. Grassi
in J Exp Med (2002) vol. 195 pp1585-97
On the brink of becoming a T cell
C. Borowski, C. Martin, F. Gounari, L. Haughn, I. Aifantis, F. Grassi, H. von Boehmer
in Curr Opin Immunol (2002) vol. 14 pp200-6
Identification of a promiscuous T-cell epitope in Mycobacterium tuberculosis Mce proteins
M. Panigada, T. Sturniolo, G. Besozzi, M. G. Boccieri, F. Sinigaglia, G. G. Grassi, F. Grassi
in Infect Immun (2002) vol. 70 pp79-85
A role for CD8 in limiting degeneracy of thymocyte selection
E. Barbier, P. A. Cazenav, F. Grassi
in Cell Mol Biol (Noisy-le-grand) (2001) vol. 47 pp129-33
Different initiation of pre-TCR and gammadeltaTCR signalling
C. Saint-Ruf, M. Panigada, O. Azogui, P. Debey, H. von Boehmer, F. Grassi
in Nature (2000) vol. 406 pp524-7
The impact of pre-T-cell receptor signals on gene expression in developing T cells
H. von Boehmer, I. Aifantis, O. Azogui, C. Saint-Ruf, F. Grassi
in Cold Spring Harb Symp Quant Biol (1999) vol. 64 pp283-9
Molecular and cellular aspects of induced thymus development in recombinase-deficient mice
S. Porcellini, M. Panigada, F. Grassi
in Eur J Immunol (1999) vol. 29 pp2476-83
The lack of chromosomal protein Hmg1 does not disrupt cell growth but causes lethal hypoglycaemia in newborn mice
S. Calogero, F. Grassi, A. Aguzzi, T. Voigtlander, P. Ferrier, S. Ferrari, M. E. Bianchi
in Nat Genet (1999) vol. 22 pp276-80
GKLF in thymus epithelium as a developmentally regulated element of thymocyte-stroma cross-talk
M. Panigada, S. Porcellini, F. Sutti, L. Doneda, O. Pozzoli, G. G. Consalez, M. Guttinger, F. Grassi
in Mech Dev (1999) vol. 81 pp103-13
Surface expression and functional competence of CD3-independent TCR zeta-chains in immature thymocytes
F. Grassi, E. Barbier, S. Porcellini, H. von Boehmer, P. A. Cazenave
in J Immunol (1999) vol. 162 pp2589-96
Expression pattern of the epithelial v-like antigen (Eva) transcript suggests a possible role in placental morphogenesis
T. Teesalu, F. Grassi, M. Guttinger
in Dev Genet (1998) vol. 23 pp317-23
Epithelial V-like antigen (EVA), a novel member of the immunoglobulin superfamily, expressed in embryonic epithelia with a potential role as homotypic adhesion molecule in thymus histogenesis
M. Guttinger, F. Sutti, M. Panigada, S. Porcellini, B. Merati, M. Mariani, T. Teesalu, G. G. Consalez, F. Grassi
in J Cell Biol (1998) vol. 141 pp1061-71
Early degenerate selection of thymocytes by class I major histocompatibility complex
F. Grassi, E. Barbier, P. A. Cazenave
in Eur J Immunol (1994) vol. 24 pp627-34
Intrathymic development of mouse alfa-beta and gamma-delta lympyhocytes
F. Grassi, P. A. Cazenave, D. Rueff-Juy
in Bullettin de l'Institut Pasteur (1994) vol. 92 pp197-211
Human immunodeficiency virus type 1 gp120 C5 region mimics the HLA class I alpha 1 peptide-binding domain
L. Lopalco, C. De Santis, R. Meneveri, R. Longhi, E. Ginelli, F. Grassi, A. G. Siccardi, A. Beretta
in Eur J Immunol (1993) vol. 23 pp2016-21
Human immunodeficiency virus type 1 gp120 mimics a hidden monomorphic epitope borne by class I major histocompatibility complex heavy chains
F. Grassi, R. Meneveri, M. Gullberg, L. Lopalco, G. B. Rossi, P. Lanza, C. De Santis, G. Brattsand, S. Butto, E. Ginelli, et al.
in J Exp Med (1991) vol. 174 pp53-62
Chemical residues of ganglioside molecules involved in interactions with lymphocyte surface targets leading to CD4 masking and inhibition of mitogenic proliferation
F. Grassi, L. Lopalco, P. Lanza, F. Ciccomascolo, F. Cazzola, A. Di Martino, G. Kirschner, L. Callegaro, L. Chieco-Bianchi, A. G. Siccardi
in Eur J Immunol (1990) vol. 20 pp145-50
Idiotypic replica of an anti-human tumor-associated antigen monoclonal antibody. Analysis of monoclonal Ab1 and Ab3 fine specificity
G. Viale, G. Flamini, F. Grassi, R. Buffa, P. G. Natali, M. Pelagi, F. Leoni, S. Menard, A. G. Siccardi
in J Immunol (1989) vol. 143 pp4338-44
Anti-human tumor antibodies induced in mice and rabbits by "internal image" anti-idiotypic monoclonal immunoglobulins
G. Viale, F. Grassi, M. Pelagi, R. Alzani, S. Menard, S. Miotti, R. Buffa, A. Gini, A. G. Siccardi
in J Immunol (1987) vol. 139 pp4250-5
HIV env glycoprotein shares a cross-reacting epitope with a surface protein present on activated human monocytes and involved in antigen presentation
A. Beretta, F. Grassi, M. Pelagi, A. Clivio, C. Parravicini, G. Giovinazzo, F. Andronico, L. Lopalco, P. Verani, S. Butto, et al.
in Eur J Immunol (1987) vol. 17 pp1793-8
Effect of beta-lactam antibiotics on migration and bactericidal activity of human phagocytes
A. Fietta, F. Sacchi, C. Bersani, F. Grassi, P. Mangiarotti, G. G. Grassi
in Antimicrob Agents Chemother (1983) vol. 23 pp930-1
Defective bactericidal reaction by the alternative pathway of complement in atopic patients
A. G. Siccardi, A. Fortunato, M. Marconi, C. Dos Santos, F. Grassi, F. Sacchi, A. Fietta
in Infect Immun (1981) vol. 33 pp710-3
Serum bacterial reaction by the alternative pathway of complement: potentiation by target multiplicity and modulation by serum factors
A. Fietta, A. Fortunato, C. Dos Santos, F. Grassi, A. G. Siccardi
in Infect Immun (1981) vol. 33 pp704-9